Size-dependent enhancement on conjugative transfer of antibiotic resistance genes by micro/nanoplastics

Recently micro/nanoplastics (MNPs) have raised intensive concerns due to their possible enhancement effect on the dissemination of antibiotic genes. Unfortunately, data is still lacking to verify the effect. In the study, the influence of polystyrene MNPs on the conjugative gene transfer was studied by using E. coli DH5alpha with RP4 plasmid as the donor bacteria and E. coli K12 MG1655 as the recipient bacteria. We found that influence of MNPs on gene transfer was size-dependent. Small MNPs (10 nm in radius) caused an increase and then a decrease in gene transfer efficiency with their concentration increasing. Moderate-sized MNPs (50 nm in radius) caused an increase in gene transfer efficiency. Large MNPs (500 nm in radius) had almost no influence on gene transfer. The gene transfer could be further enhanced by optimizing mating time and mating ratio. Scavenging reactive oxygen species (ROS) production did not affect the cell membrane permeability, indicating that the increase in cell membrane permeability was not related to ROS production. The mechanism of the enhanced gene transfer efficiency was attributed to a combined effect of the increased ROS production and the increased cell membrane permeability, which ultimately regulated the expression of corresponding genes.

Automated summary

In this study, the influence of polystyrene micro/nanoplastics (MNPs) on gene transfer was investigated. It was found that the effect of MNPs on gene transfer was dependent on their size, with small MNPs causing a fluctuation in gene transfer efficiency, moderate-sized MNPs increasing gene transfer efficiency, and large MNPs having minimal impact. Furthermore, the gene transfer efficiency could be further enhanced by optimizing mating time and ratio.