Journal
JOURNAL OF FISH DISEASES
Volume 45, Issue 3, Pages 435-443Publisher
WILEY
DOI: 10.1111/jfd.13572
Keywords
Danio rerio; fish mycobacteriosis; genetic lines; Mycobacterium chelonae; zebrafish
Funding
- Office of Research Infrastructure Programs of the National Institutes of Health (NIH) [R24OD010998]
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This study found differences in severity of Mycobacterium infections among four different zebrafish lines commonly used in research, but no significant differences in prevalence.
Mycobacteriosis is one of the most common diseases encountered in laboratory zebrafish. These infections can present a problem to researchers using zebrafish because they may introduce unknown experimental variables. Whilst differences in severity of infections between species of Mycobacterium infecting zebrafish have been well documented, little is known about differences in susceptibility between zebrafish lines. Previous surveys have found higher prevalence in the TU zebrafish line relative to other lines, suggesting that there may be underlying genetic differences in susceptibility. This study investigates Mycobacterium chelonae H1E2-GFP infections in four different zebrafish lines commonly used in research (AB, 5D, casper and TU). Fish were exposed to a labelled (green-fluorescent protein (GFP)) strain of M. chelonae by intraperitoneal injection, and infection status was evaluated after 10 weeks. Visualization of GFP in euthanized fish and histology were used as endpoints. In GFP images, severity was assessed by image analysis, and in histological sections, counts of granulomas containing acid-fast bacteria were used. Results indicated differences in severity of infections between lines, but no significant differences in prevalence.
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