4.7 Article

Fungal oxysterol-binding protein-related proteins promote pathogen virulence and activate plant immunity

Journal

JOURNAL OF EXPERIMENTAL BOTANY
Volume 73, Issue 7, Pages 2125-2141

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/jxb/erab530

Keywords

Arabidopsis; damage-associated molecular pattern; fungal development; fungal virulence; Magnaporthe oryzae; oxysterol-binding protein; pathogen-associated molecular pattern; plant immunity; rice blast disease

Categories

Funding

  1. National Natural Science Foundation of China [31872917]

Ask authors/readers for more resources

Oxysterol-binding protein-related proteins (ORPs) play important roles in plant-pathogen interactions, triggering oxidative bursts and promoting plant innate immunity; they are also essential for the growth, development, and virulence of fungal pathogens.
Oxysterol-binding protein-related proteins (ORPs) are a conserved class of lipid transfer proteins that are closely involved in multiple cellular processes in eukaryotes, but their roles in plant-pathogen interactions are mostly unknown. We show that transient expression of ORPs of Magnaporthe oryzae (MoORPs) in Nicotiana benthamina plants triggered oxidative bursts and cell death; treatment of tobacco Bright Yellow-2 suspension cells with recombinant MoORPs elicited the production of reactive oxygen species. Despite ORPs being normally described as intracellular proteins, we detected MoORPs in fungal culture filtrates and intercellular fluids from barley plants infected with the fungus. More importantly, infiltration of Arabidopsis plants with recombinant Arabidopsis or fungal ORPs activated oxidative bursts, callose deposition, and PR1 gene expression, and enhanced plant disease resistance, implying that ORPs may function as endogenous and exogenous danger signals triggering plant innate immunity. Extracellular application of fungal ORPs exerted an opposite impact on salicylic acid and jasmonic acid/ethylene signaling pathways. Brassinosteroid Insensitive 1-associated Kinase 1 was dispensable for the ORP-activated defense. Besides, simultaneous knockout of MoORP1 and MoORP3 abolished fungal colony radial growth and conidiation, whereas double knockout of MoORP1 and MoORP2 compromised fungal virulence on barley and rice plants. These observations collectively highlight the multifaceted role of MoORPs in the modulation of plant innate immunity and promotion of fungal development and virulence in M. oryzae. Oxysterol-binding protein-related proteins are novel immunogenic pattern molecules that are required for the growth, development, and virulence of the fungal pathogen Magnaporthe oryzae.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available