Maintaining osmotic balance in legume nodules

Pinitol (3-O-methyl-d-chiro-inositol) is shown to be transported passively across the plant-derived symbiosome membrane in Lotus japonicus by the energy-independent polyol transporter LjPLT11. Accumulation of pinitol is crucial to maintenance of osmotic balance in nodules. RNAi lines reduced in LjPLT11 have disrupted symbiosome membranes, reduced N-2 fixation by rhizobial bacteroids and reduced plant growth. There is also an increase in production of reactive oxygen species (ROS) in LjPLT11 RNAi lines, consistent with osmotic imbalance causing increased stress in nodules.

Automated summary

Pinitol is transported passively across the symbiosome membrane in Lotus japonicus by the energy-independent polyol transporter LjPLT11. Its accumulation is crucial for maintaining osmotic balance in nodules. The disruption of symbiosome membranes, reduced nitrogen fixation, and increased production of reactive oxygen species in LjPLT11 RNAi lines indicate the importance of LjPLT11 in nodules.