Journal
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
Volume 37, Issue 1, Pages 451-461Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14756366.2021.2018682
Keywords
Oleanolic acid; structural modification; alpha-glucosidase; alpha-amylase; enzyme inhibition
Funding
- National Natural Science Foundation of China [81803390]
- Natural Science Foundation of Guangdong Province [2021A1515010221]
- Special Fund Project of Science and Technology Innovation Strategy of Guangdong Province [Jiangke (2018)352, Jiangke (2020)182]
- Department of Education of Guangdong Province [2017KSYS010, 2019KZDXM035, 2021KCXTD044, 2021KTSCX135]
- Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds) [pdjh2021a0504]
- Jiangmen City Science and Technology Basic Research Project [2020030101030005457]
Ask authors/readers for more resources
Different oleanolic add (OA) oxime ester derivatives were designed and synthesised to develop inhibitors against alpha-glucosidase and 7 amylase. Compound 3a showed the highest alpha-glucosidase inhibition, while compound 3f exhibited the highest alpha-amylase inhibitory. Both compounds showed a good safety profile against cells.
Different oleanolic add (OA) oxime ester derivatives (3a-3t) were designed and synthesised to develop inhibitors against alpha-glucosidase and 7 amylase. An the synthesised OA derivatives were evaluated against alpha-glucosidase and a-amylase in vitro. Among them, compound 3a showed the highest alpha-glucosidase inhibition with an IC(50 )of 0.35 mu M, which was, similar to 1900 times stronger than that of acarbose, meanwhile compound 3f exhibited the highest 2-amylase inhibitory with an IC50 of 3.80 mu M that was similar to 26 times higher than that of acarbose. The inhibition kinetic studies showed that the inhibitory mechanism of compounds 3a and 3f were reversible and mixed types towards alpha-glucosidase and alpha-amylase, respectively. Molecular docking studies analysed the interaction between compound and two enzymes, respectively. Furthermore, cytotoxicity evaluation assay demonstrated a high level of safety profile of compounds 3a and 3f against 3T3-L1 and HepG2 cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available