Journal
JOURNAL OF CLEANER PRODUCTION
Volume 328, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jclepro.2021.129437
Keywords
Laccase; Malachite green; Degradation; Detoxification; High temperature
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Funding
- Scientific and Technological Project of Henan Province [192102310245]
- National Natural Science Foundation of China [31800050]
- Zhongyuan Thousand Talents Project [204200510018]
- Fundamental Research Funds for the Central Universities [B200201055]
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The novel DUF152 laccase Cblac Mut8 was designed for enhanced thermostability and successfully degraded and detoxified the toxic dye malachite green at high temperatures. Systematic toxicity assessments showed Mut8 thoroughly eliminated the toxicity to bacteria, Zea mays, and zebrafish from the degradation products.
Laccases are green biocatalysts that are advantageous in the bioremediation of the triphenylmethane dye malachite green (MG). Here, Cblac from Caldicellulosiruptor bescii was characterized to be a novel DUF152 laccase. Cblac mutant Mut8 was designed with enhanced thermostability, which was demonstrated by the Thermofluor assay, circular dichroism spectrum, and structural analyses. MG bioremediation by Mut8 at high temperatures was performed and examined for its potential to degrade and detoxify the high-temperature MG effluent. Approximately 100% of 100 mg/L of MG was degraded by 40 U/L of Mut8 within 2 h at 75 degrees C. Mut8 initiated the degradation via oxidative cleavage of the central C-C bond and N-demethylation of MG, and the intermediates were further demethylated, hydroxylated and oxidized. Moreover, the systematic toxicity assessment of the MG degradation products using bacteria, Zea mays, and zebrafish demonstrated that Mut8 thoroughly eliminated MG toxicity. Taken together, Mut8 could effectively decolorize and detoxify MG at high temperatures, showing great promise as an environmental friendly and effective approach to remediate MG effluent immediately released from the dyeing process, thus avoiding the cooling process and lowering the cost simultaneously.
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