4.5 Article

Europium (III) chelate nanoparticle-based lateral flow immunoassay strips for rapid and quantitative detection of cystatin C in serum

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ELSEVIER
DOI: 10.1016/j.jchromb.2022.123133

Keywords

Renal failure; Glomerular filtration rate; Cystatin C; Europium-chelate nanoparticles; Lateral flow immunoassay

Funding

  1. National Natural Science Foundation of China [81702072]

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In this study, a Eu (III) chelate nanoparticles based lateral flow immunoassay was developed to achieve real-time monitoring of Cys C concentration in serum within 15 min. This method showed a wide detection range and a low limit of detection, and exhibited satisfactory correlation with chemiluminescence immunoassay.
The concentration of Cys C in the patient's serum can reflect the level of glomerular filtration rate and indicate the occurrence of renal failure. The establishment of a simple and rapid analytical method to quantitatively monitor the concentration of Cys C in serum could help timely detection of renal failure. In this study, we have developed an Eu (III) chelate nanoparticles based lateral flow immunoassay to fulfill real-time monitoring of Cys C concentration in serum within 15 min. This method was performed as a sandwich immunoassay with a wide detection range (0.05-10 mu g/mL) and a low limit of detection (24.54 ng/mL). The intra and inter-assay coefficients of variation were 8.31-8.61% and 8.92-9.95%, respectively. Furthermore, the application of this method was evaluated by comparing the determined results with those obtained by chemiluminescence immunoassay, exhibiting a satisfactory correlation (R-2 = 0.9830). The developed LFIA method with satisfactory analytical performance has great potential for real-time monitoring of renal failure and self-detection for the high-risk population.& nbsp;& nbsp;

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