Somatostatin immunoreactivity within the urinary bladder nerve fibers and paracervical ganglion urinary bladder projecting neurons in the female pig

The study was designed to examine the distribution and chemical coding of somatostatin-immunoreactive (SOMIR) nerve fibers supplying the urinary bladder wall and to establish the distribution and immunohistochemical characteristics of the subpopulation of paracervical ganglion (PCG) SOM-IR neurons projecting to this organ in female pigs. The PCG-urinary bladder projecting neurons (PCG-UBPN) were visualized with retrograde neuronal tracer Fast Blue (FB). Double-labeling immunohistochemistry performed on cryostat sections from the urinary bladder wall revealed that the greatest density of SOM-IR nerve fibers was found in the muscle layer and around blood vessels, a moderate number of these nerve terminals supplied the submucosa and only single SOM-IR axons were encountered beneath the urothelium. In all the investigated sections the vast majority of SOM-IR nerve fibers were immunopositive to vesicular acetylcholine transporter (VAChT) and many SOM-IR axons contained immunoreactivity to neuropeptide Y (NPY). Approximately 65 % of FB-positive (FB+) PCG-UBPN were immunoreactive to SOM. Moreover, PCG FB+/SOM + nerve cells were simultaneously immunoreactive to choline acetyltransferase (ChAT; 64.6 +/- 0.6 %), NPY (59.7 +/- 1.2 %), neuronal nitric oxide synthase (nNOS; 46.1 +/- 0.7 %), vasoactive intestinal polypeptide (VIP; 29.9 +/- 2.2 %), Leu5-enkephalin (L-ENK; 19.5 +/- 6.3 %), dopamine beta-hydroxylase (D beta H; 14.9 +/- 1.9 %) or pituitary adenylate cyclase-activating polypeptide (PACAP; 14.8 +/- 2.4 %). The present study reveals the extensive expression of SOM in both the nerve fibres supplying the porcine urinary bladder wall and the PCG neurons projecting to this organ, indicating an important regulatory role of SOM in the control of the urinary bladder function.

Automated summary

The study examined the distribution and chemical coding of somatostatin-immunoreactive (SOMIR) nerve fibers supplying the urinary bladder wall and the paracervical ganglion (PCG) neurons projecting to this organ in female pigs. It was found that somatostatin is extensively expressed in both the nerve fibers supplying the porcine urinary bladder wall and the PCG neurons projecting to this organ, indicating an important regulatory role of SOM in the control of the urinary bladder function.