Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 26, Issue 7, Pages 2063-2075Publisher
WILEY
DOI: 10.1111/jcmm.17229
Keywords
aqueous humour; cell survival; glaucoma; ocular hypertension; trabecular meshwork; vasorin
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Funding
- National Eye Institute [R01-EY028823, R01EY018590]
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In this study, we found that vasorin expression levels were lower in the aqueous humour of glaucoma patients. The research also indicated the important role of vasorin in maintaining cell survival and inhibiting cellular responses induced by TGF-beta. These findings reveal the potential role of vasorin in the pathobiology of glaucoma.
Glaucoma, one of the leading causes of irreversible blindness, is commonly associated with elevated intraocular pressure due to impaired aqueous humour (AH) drainage through the trabecular meshwork. The aetiological mechanisms contributing to impaired AH outflow, however, are poorly understood. Here, we identified the secreted form of vasorin, a transmembrane glycoprotein, as a common constituent of human AH by mass spectrometry and immunoblotting analysis. ELISA assay revealed a significant but marginal decrease in vasorin levels in the AH of primary open-angle glaucoma patients compared to non-glaucoma cataract patients. Human trabecular meshwork (HTM) cells were confirmed to express vasorin, which has been shown to possess anti-apoptotic and anti-TGF-beta activities. Treatment of HTM cells with vasorin induced actin stress fibres and focal adhesions and suppressed TGF-beta 2-induced SMAD2/3 activation in HTM cells. Additionally, cobalt chloride-induced hypoxia stimulated a robust elevation in vasorin expression, and vasorin suppressed TNF-alpha-induced cell death in HTM cells. Taken together, these findings reveal the importance of vasorin in maintenance of cell survival, inhibition of TGF-beta induced biological responses in TM cells, and the decreasing trend in vasorin levels in the AH of glaucoma patients suggests a plausible role for vasorin in the pathobiology of ocular hypertension and glaucoma.
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