Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 25, Issue 22, Pages 10747-10760Publisher
WILEY
DOI: 10.1111/jcmm.17016
Keywords
adipose tissue; umbilical cord; bone marrow; cell therapy; mesenchymal stem; stromal cells; preservation condition; Ringer lactate; sodium chloride
Categories
Funding
- Vingroup Scientific and Clinical Research Grant [PRO.19.47, PRO.19.48, PRO.19.49]
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The interaction between mesenchymal stem/stromal cells (MSCs) and preservation conditions plays a crucial role in maintaining cell viability and functionality. Different types of MSCs showed varying viability and metabolic profiles under different storage conditions. The cells released various growth factors, cytokines, and cytokines during preservation, maintaining their stemness and functionality.
The interplay between mesenchymal stem/stromal cells (MSCs) and preservation conditions is critical to maintain the viability and functionality of these cells before administration. We observed that Ringer lactate (RL) maintained high viability of bone marrow-derived MSCs for up to 72 h at room temperature (18 degrees C-22 degrees C), whereas adipose-derived and umbilical cord-derived MSCs showed the highest viability for 72 h at a cold temperature (4 degrees C-8 degrees C). These cells maintained their adherence ability with an improved recovery rate and metabolic profiles (glycolysis and mitochondrial respiration) similar to those of freshly harvested cells. Growth factor and cytokine analyses revealed that the preserved cells released substantial amounts of leukaemia inhibitory factors (LIFs), hepatocyte growth factor (HGF) and vascular endothelial growth factor-A (VEGF-A), as well as multiple cytokines (eg IL-4, IL-6, IL-8, MPC-1 and TNF-alpha). Our data provide the simplest clinically relevant preservation conditions that maintain the viability, stemness and functionality of MSCs from perinatal and adult tissue sources.
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