4.5 Article

Peroxisomal support of mitochondrial respiratory efficiency promotes ER stress survival

Journal

JOURNAL OF CELL SCIENCE
Volume 135, Issue 1, Pages -

Publisher

COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.259254

Keywords

Endoplasmic reticulum; Mitochondria; Stress surviva; Peroxisome; Reactive oxygen species

Categories

Funding

  1. University of Michigan Protein Folding Disease Initiative
  2. National Institutes of Health [R21 AG058862]

Ask authors/readers for more resources

Endoplasmic reticulum stress (ERS) occurs when cellular demand for protein folding exceeds the capacity of the organelle. Adaptation and cell survival in response to ERS require the contribution of mitochondria and peroxisomes. Our study in yeast shows that during ERS, both mitochondrial respiration and peroxisome abundance increase to promote an adaptive response.
Endoplasmic reticulum stress (ERS) occurs when cellular demand for protein folding exceeds the capacity of the organelle. Adaptation and cell survival in response to ERS requires a critical contribution by mitochondria and peroxisomes. During ERS responses, mitochondrial respiration increases to ameliorate reactive oxygen species (ROS) accumulation. We now show in yeast that peroxisome abundance also increases to promote an adaptive response. In pox1 Delta cells, which are defective in peroxisomal beta-oxidation of fatty acids, the respiratory response to ERS is impaired and ROS accrues. However, the respiratory response to ERS is rescued and ROS production is mitigated in pox1 Delta cells overexpressing Mpc1, the mitochondrial pyruvate carrier that provides another source of acetyl CoA to fuel the tricarboxylic acid cycle and oxidative phosphorylation. Using proteomics, select mitochondrial proteins were identified that undergo upregulation upon ERS to remodel the respiratory machinery. The abundance of several peroxisome-based proteins was also increased, corroborating the role of peroxisomes in ERS adaptation. Finally, ERS stimulates assembly of respiratory complexes into higher-order supercomplexes, underlying increased electron transfer efficiency. Our results highlight peroxisomal and mitochondrial support for ERS adaptation to favor cell survival.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available