Journal
JOURNAL OF CARDIOTHORACIC SURGERY
Volume 16, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13019-021-01698-2
Keywords
miR-497-5p; CDCA4; LUSC; Proliferation; Migration; Invasion
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MiR-497-5p was found to be expressed at low levels in LUSC tissue and cells. Overexpression of miR-497-5p significantly inhibited cancer progression by targeting CDCA4, thereby restraining the development of LUSC.
Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.
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