Journal
JOURNAL OF BIOTECHNOLOGY
Volume 341, Issue -, Pages 137-145Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2021.09.018
Keywords
Monascus pigments; Citrinin; Cell growth; Triton X-100; Extractive fermentation
Categories
Funding
- Research and Development Plan in Key Areas of Hunan Province [2019NK2111]
- Natural Sci-ence Foundation of Hunan Province [2019JJ40542]
- Changsha Key S&T Special Projects [kh2003013]
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The addition of 3 g/L Triton X-100 significantly increases the production of Monascus pigments and citrinin in submerged fermentation by M. purpureus DK, leading to enhanced biosynthesis of secondary metabolites through upregulation of gene expression levels.
Extractive fermentation has been proven to be efficient in enhancing the secretion and production of secondary metabolites in submerged fermentation by Monascus spp., owing to increased cell membrane permeability and resolved product inhibition. In this study, we investigated the regulation effect of Triton X-100 on cell growth and secondary metabolite biosynthesis in submerged fermentation of M. purpureus DK. The results show that the maximum monascus pigments (MPs), citrinin (CIT) production, and specific growth rate are 136.86 U/mL, 4.57 mg/L, and 0.04 h(-1), respectively, when 3 g/L of Triton X-100 is supplemented after fermentation for 10 d, and the extracellular MPs and CIT increased by 127.48% and 288.57%, respectively. RT-qPCR shows that the expression levels of MPs and CIT biosynthesis gene clusters are significantly upregulated, whereas those of glycolysis, tricarboxylic acid cycle, respiratory chains, and ATP synthase are downregulated. This study provides a vital strategy for extractive fermentation under extreme environmental conditions for further enhancing MP production.
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