Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 26, Issue 11, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.26.11.116004
Keywords
quantitative phase imaging; frequency comb; cardiovascular dynamics; zebrafish; high-speed phase measurement
Funding
- National Research Foundation of the Republic of Korea [NRF-2012R1A3A1050386, NRF-2020R1A2C2102338, NRF-2020R1A2C210233811, NRF-2021R1A4A1031660]
- KAIST UP Program
- Singapore National Research Foundation [NRF-NRFF2015-02]
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This study successfully measured heart rate and blood flow in zebrafish embryos using FCR-QPI technique, revealing the average velocity of RBCs and heart rate at different stages of development.
Significance: Real-time monitoring of the heart rate and blood flow is crucial for studying cardiovascular dysfunction, which leads to cardiovascular diseases. Aim: This study aims at in-depth understanding of high-speed cardiovascular dynamics in a zebrafish embryo model for various biomedical applications via frequency-comb-referenced quantitative phase imaging (FCR-QPI). Approach: Quantitative phase imaging (QPI) has emerged as a powerful technique in the field of biomedicine but has not been actively applied to the monitoring of circulatory/cardiovascular parameters, due to dynamic speckles and low frame rates. We demonstrate FCR-QPI to measure heart rate and blood flow in a zebrafish embryo. FCR-QPI utilizes a high-speed photodetector instead of a conventional camera, so it enables real-time monitoring of individual red blood cell (RBC) flow. Results: The average velocity of zebrafish' s RBCs was measured from 192.5 to 608.8 mu m/s at 24 to 28 hour-post-fertilization (hpf). In addition, the number of RBCs in a pulsatile blood flow was revealed to 16 cells/pulse at 48 hpf. The heart rates corresponded to 94 and 142 beats-per-minute at 24 and 48 hpf. Conclusions: This approach will newly enable in-depth understanding of the cardiovascular dynamics in the zebrafish model and possible usage for drug discovery applications in biomedicine. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License.
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