A Study of Hydrophobically Modified Pullulan Nanoparticles with Different Hydrophobic Densities on the Effect of Anti-Colon Cancer Cell Efficiency

To discuss the effect of hydrophobic groups of a polymer on the structural properties and function of polymer nanoparticles (NPs), we grafted chenodeoxycholic acid (CDCA) with pullulan (PU) to form hydrophobically modified PU (PUC). Three PUC polymers, namely, PUC-1, PUC-2, and PUC-3, with different degrees of substitution were designed by changing the feed ratio of CDCA and PU. 1H-NMR spectra showed that the PUC polymer was successfully synthesized, and the degrees of hydrophobic substitution for PUC-1, PUC-2, and PUC-3 were calculated to be 10.66%, 13.92%, and 16.94%, respectively. The PUC NPs were prepared by the dialysis method and were shown to be uniformly spherical by transmission electron microscopy (TEM).IP: The127.0.0.1average On: sizesFri, 29were Oct about2021 (220 +/- 05:25:28 10) nm, (203 +/- 7) nm, and (163 +/- 6) nm under dynamic light scattering (DLS) for PUC-1 NPs, PUC-2 NPs, ad PUC-3 NPs, espectively. Drug release experiments Copyright: American Scent fic Publishers showed that the three PUC/DOX NPs exhibited good sustained release. At 48 h, the IC50 of doxorubicin in inhibiting colon Delivered by Ingena cancer HCT116 cells was 0.0904 mu g/mL. A cell study showed that PUC-3/DOX NPs had the highest uptake efficiency by HCT116 cells with the most cytotoxicity and inhibited the migration of HCT116 cells with the highest efficiency. The structural properties and function of polymer NPs were closely related to the hydrophobic groups in the polymer, and NPs with higher hydrophobicity showed a smaller size, higher drug capacity, and greater cell efficiency.

Automated summary

The study investigated the impact of hydrophobic groups on the structural properties and function of polymer nanoparticles. The results demonstrated that polymer NPs with higher hydrophobicity exhibited smaller size, higher drug capacity, and greater cell efficiency.