Enhanced antibacterial properties on superhydrophobic micro-nano structured titanium surface

Micro/nano scale surface modifications of titanium based orthopedic and cardiovascular implants has shown to augment biocompatibility. However, bacterial infection remains a serious concern for implant failure, aggravated by increasing antibiotic resistance and over usage of antibiotics. Bacteria cell adhesion on implant surface leads to colonization and biofilm formation resulting in morbidity and mortality. Hence, there is a need to develop new implant surfaces with high antibacterial properties. Recent developments have shown that superhydrophobic surfaces prevent protein and bacteria cell adhesion. In this study, a thermochemical treatment was used modify the surface properties for high efficacy antibacterial activity on titanium surface. The modification led to a micro-nano surface topography and upon modification with polyethylene glycol (PEG) and silane the surfaces were superhydrophilic and superhydrophobic, respectively. The modified surfaces were characterized for morphology, wettability, chemistry, corrosion resistance and surface charge. The antibacterial capability was characterized with Staphylococcus aureus and Escherichia coli by evaluating the bacteria cell inhibition, adhesion kinetics, and biofilm formation. The results indicated that the superhydrophobic micro-nano structured titanium surface reduced bacteria cell adhesion significantly (>90%) and prevented biofilm formation compared to the unmodified titanium surface after 24 h of incubation.

Automated summary

Micro/nano scale surface modifications of titanium based orthopedic and cardiovascular implants have been shown to improve biocompatibility. However, bacterial infection remains a major concern for implant failure. Recent studies have found that superhydrophobic surfaces can prevent protein and bacterial cell adhesion. This study used a thermochemical treatment to modify the properties of the titanium surface and achieved superhydrophilic and superhydrophobic surfaces by modifying with polyethylene glycol and silane, respectively. The modified surfaces significantly reduced bacterial cell adhesion and prevented biofilm formation.