Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 33, Issue 6, Pages 769-782Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2021.2019366
Keywords
Duck feet collagen; hydroxyapatite; sponge; bone marrow stem cell; bone regeneration
Funding
- International Research & Development Program of the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2021K1A3A1A78097905]
- National Research Foundation of Korea [2021K1A3A1A78097905] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study focused on collagen extracted from duck's feet as a potential scaffold for bone tissue implantation. The experiment showed that the duck's feet-derived collagen had good cell affinity and low rejection, making it suitable for bone graft materials in tissue engineering applications. Overall, the DC/HAp sponges demonstrated biocompatibility and promising physical properties for bone regeneration.
Collagen, a natural biomaterial derived from animal tissues, has attracted the attention of biomedical material researchers because of its excellent cell affinity and low rejection in vivo. In this study, collagen was extracted using livestock by-product flippers, and an experiment was performed to assess its application as a scaffold for bone tissue implantation. For this purpose, we fabricated 2%, and 3% duck's feet derived collagen (DC) sponges. We then compared them to hydroxyapatite (HAp)-coated DC sponges, and measured the porosity and pore size using scanning electron microscopy (SEM) to analyze the physical properties and morphology of DC and DC/HAp sponges. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were carried out to measure the proliferation of bone marrow stem cells (BMSCs) in DC and DC/HAp sponges. An alkaline phosphatase activity assay confirmed the osteogenic differentiation ability of BMSCs. Polymerase chain reaction (PCR) was performed to confirm the BMSC-specific genetic marker. The osteogenic potential was confirmed by the bone formation in an in vivo environment on the scaffold by histological and immunohistochemical analysis. Overall, this study shows that DC/HAp sponges have biocompatibility and good physical properties. Additionally, DC/HAp sponges show potential use as bone graft materials for tissue engineering applications.
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