Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 27, Issue 2, Pages 249-260Publisher
SPRINGER
DOI: 10.1007/s00775-022-01930-x
Keywords
Europium; Buffer; TRLFS; NMR; Stability constant
Funding
- Projekt DEAL
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The interaction between Eu(III) ion and different pH buffers was studied using NMR and TRLFS techniques. The results showed that Good's buffers have significant interaction with Eu(III), while TRIS buffer has practically no affinity towards Eu(III). Therefore, TRIS buffer should be preferred when investigating lanthanides.
The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good's buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good's buffers. [GRAPHICS] .
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