4.6 Article

Investigation of sugar binding kinetics of the E. coli sugar/H+ symporter XylE using solid-supported membrane-based electrophysiology

Related references

Note: Only part of the references are listed.
Article Biophysics

SSM-based electrophysiology, a label-free real-time method reveals sugar binding & transport events in SGLT1

Andre Bazzone et al.

Summary: SSM-based electrophysiology is a powerful technique for studying sugar binding and Na+/glucose cotransport by SGLT1, providing real-time current readouts and insights into substrate-induced electrogenic events. This approach allows for the observation of conformational transitions, overcoming limitations seen in other techniques and offering new perspectives for functional studies of transporters.

BIOSENSORS & BIOELECTRONICS (2022)

Review Biotechnology & Applied Microbiology

Biochemical routes for uptake and conversion of xylose by microorganisms

Zhe Zhao et al.

BIOTECHNOLOGY FOR BIOFUELS (2020)

Article Multidisciplinary Sciences

pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

Andre Bazzone et al.

PLOS ONE (2016)

Review Biophysics

Structural Biology of the Major Facilitator Superfamily Transporters

Nieng Yan

ANNUAL REVIEW OF BIOPHYSICS, VOL 44 (2015)

Article Multidisciplinary Sciences

Molecular basis of ligand recognition and transport by glucose transporters

Dong Deng et al.

NATURE (2015)

Article Multidisciplinary Sciences

Transient conformers of LacY are trapped by nanobodies

Irina Smirnova et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site

Hemant Kumar et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

A chemiosmotic mechanism of symport

H. Ronald Kaback

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Crystal structure of the human glucose transporter GLUT1

Dong Deng et al.

NATURE (2014)

Article Multidisciplinary Sciences

Substrate-induced changes in the structural properties of LacY

Tetiana Serdiuk et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Multidisciplinary Sciences

Structure of sugar-bound LacY

Hemant Kumar et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Multidisciplinary Sciences

Functional architecture of MFS D-glucose transporters

M. Gregor Madej et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Multidisciplinary Sciences

Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE

Goragot Wisedchaisri et al.

NATURE COMMUNICATIONS (2014)

Article Biochemistry & Molecular Biology

Electrophysiological Characterization of Uncoupled Mutants of LacY

Olga Gaiko et al.

BIOCHEMISTRY (2013)

Article Physiology

Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

Juan Garcia-Celma et al.

JOURNAL OF GENERAL PHYSIOLOGY (2013)

Article Biochemistry & Molecular Biology

Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters

Esben M. Quistgaard et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2013)

Article Multidisciplinary Sciences

Evolutionary mix-and-match with MFS transporters

M. Gregor Madej et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Review Biochemistry & Molecular Biology

Cooperativity in monomeric enzymes with single ligand-binding sites

Carol M. Porter et al.

BIOORGANIC CHEMISTRY (2012)

Article Multidisciplinary Sciences

Crystal structure of a bacterial homologue of glucose transporters GLUT1-4

Linfeng Sun et al.

NATURE (2012)

Article Multidisciplinary Sciences

Apo-intermediate in the transport cycle of lactose permease (LacY)

M. Gregor Madej et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Biochemistry & Molecular Biology

Lactose Permease and the Alternating Access Mechanism

Irina Smirnova et al.

BIOCHEMISTRY (2011)

Article Biochemistry & Molecular Biology

G117C MelB, a mutant melibiose permease with a changed conformational equilibrium

C. Ganea et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2011)

Article Biochemistry & Molecular Biology

An Early Event in the Transport Mechanism of LacY Protein INTERACTION BETWEEN HELICES V AND I

Yonggang Zhou et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Multidisciplinary Sciences

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition

Vincent Chaptal et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Biochemistry & Molecular Biology

Delineating Electrogenic Reactions during Lactose/H+ Symport

Juan J. Garcia-Celma et al.

BIOCHEMISTRY (2010)

Article Biochemistry & Molecular Biology

Helix Dynamics in LacY: Helices II and IV

Zhenyu Liu et al.

JOURNAL OF MOLECULAR BIOLOGY (2010)

Article Multidisciplinary Sciences

Structure of a fucose transporter in an outward-open conformation

Shangyu Dang et al.

NATURE (2010)

Article Biochemistry & Molecular Biology

Structural characterization of the osmosensor ProP

Wajid M. H. Sayeed et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2009)

Article Multidisciplinary Sciences

Electrophysiological characterization of LacY

Juan J. Garcia-Celma et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Article Chemistry, Multidisciplinary

Rapid activation of the melibiose permease MeIB immobilized on a solid-supported membrane

Juan J. Garcia-Celma et al.

LANGMUIR (2008)

Article Biochemical Research Methods

SSM-based electrophysiology

Patrick Schulz et al.

METHODS (2008)

Article Multidisciplinary Sciences

Sugar binding induces an outward-facing conformation of LacY

Irina Smirnova et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Multidisciplinary Sciences

Structural determination of wild-type lactose permease

Lan Guan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Biochemistry & Molecular Biology

Direct sugar binding to LacY measured by resonance energy transfer

Irina N. Smirnova et al.

BIOCHEMISTRY (2006)

Article Biochemistry & Molecular Biology

Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli

Yiling Nie et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2006)

Article Biochemistry & Molecular Biology

The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding

Kerstin Meyer-Lipp et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2006)

Article Biochemistry & Molecular Biology

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY

O Mirza et al.

EMBO JOURNAL (2006)

Article Biochemistry & Molecular Biology

Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli

D Zuber et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS (2005)

Article Multidisciplinary Sciences

Binding affinity of lactose permease is not altered by the H+ electrochemical gradient

L Guan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Article Chemistry, Multidisciplinary

UCSF chimera - A visualization system for exploratory research and analysis

EF Pettersen et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2004)

Article Multidisciplinary Sciences

Structure and mechanism of the lactose permease of Escherichia coli

J Abramson et al.

SCIENCE (2003)

Article Physics, Multidisciplinary

Universal behavior of load distribution in scale-free networks

KI Goh et al.

PHYSICAL REVIEW LETTERS (2001)

Article Physics, Fluids & Plasmas

Scientific collaboration networks. I. Network construction and fundamental results

MEJ Newman

PHYSICAL REVIEW E (2001)