Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 70, Issue 7, Pages 2303-2311Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c07646
Keywords
myrosinase; Rahnella inusitata; functional component; sulforaphene and sulforaphane
Funding
- Young Talent of Lifting Engineering for Science and Technology in Shandong, China [SDAST2021qt18]
- Natural Science Foundation of Shandong Province [ZR2020JQ15]
- Taishan Scholar Project of Shandong Province
- Fundamental Research Funds for the Central Universities [201941002]
- Key Technology Research and Development Program of Shandong [2018SDKJ0304]
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This study demonstrates that Rmyr is a highly active and stable myrosinase capable of efficiently degrading glucosinolates to sulforaphene and sulforaphane. It shows potential as a biocatalyst for large-scale preparation of these compounds.
Myrosinase is a biotechnological tool for the preparation of sulforaphane and sulforaphene with a variety of excellent biological activities. In this study, a gene encoding the novel glycoside hydrolase family 3 (GH3) myrosinase Rmyr from Rahnella inusitata was heterologously expressed in Escherichia coli BL21 (DE3). The purified Rmyr shows the highest activity at 40 degrees C and pH 7.0; meanwhile, its half-life at 30 degrees C reaches 12 days, indicating its excellent stability. Its sinigrin-, glucoraphenin-, and glucoraphanin-hydrolyzing activities were 12.73, 4.81, and 6.99 U/mg, respectively. Rmyr could efficiently degrade the radish seed-derived glucoraphenin and the broccoli seed-derived glucoraphanin into sulforaphene and sulforaphane within 10 min with the highest yields of 5.07 mg/g radish seeds and 9.56 mg/g broccoli seeds, respectively. The highest conversion efficiencies of sulforaphane from glucoraphanin and sulforaphene from glucoraphenin reached up to 92.48 and 97.84%, respectively. Therefore, Rmyr is a promising and potent biocatalyst for efficient and large-scale preparation of sulforaphane and sulforaphene.
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