Large Inter- and Intraspecies Variability of Polyphenols and Proanthocyanidins in Eight Temperate Forage Species Indicates Potential for Their Exploitation as Nutraceuticals

Substantial efforts have been made in incorporating tannin-rich forages into grassland-based livestock production systems. However, the structural and functional diversity of tannins in different species limits their potential use at the field scale. We conducted a greenhouse experiment with 17 cultivars from 8 forage species and their cultivars. Ultraperformance liquid chromatography tandem mass spectrometry was used to analyze their polyphenolic profile and proanthocyanidin (PA) structural features in leaves. Our results highlight large inter- and intraspecies variability of plants in terms of polyphenol and tannin concentrations in the leaves. A concomitant and significant variation was also registered in the structural features of PA-rich forages such as the mean degree of polymerization and prodelphinidin percentage. The concentration of PA also varied within plant organs; the highest concentration was in flowers, but leaves had the highest contribution to harvestable PA biomass. Our research highlights that identifying these variations helps in identifying the representativeness of bioactivity and provides the basis for targeted breeding programs.

Automated summary

This study revealed significant intra- and interspecific variability in polyphenol and tannin concentrations in plant leaves, as well as structural variation in PA-rich forages. Additionally, the concentration of PA was found to vary among plant organs, with flowers showing the highest concentration and leaves contributing the most to harvestable PA biomass.