Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 43, Pages 12870-12879Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c05464
Keywords
denaturation treatment; disulfide bonds; IgG/IgE-binding capacity; linear epitopes; sarcoplasmic calcium-binding protein
Funding
- National Natural Scientific Foundation of China [31871720, 32072336, 32001695, 31901811]
- National Key R&D Program of China [2019YFD0901703]
- Science and Technology Program of Xiamen City [3502Z20203059]
- Marine Scientific Research Special Foundation for Public Sector Program [DY135-B2-07]
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The study identified linear epitopes of Sarcoplasmic calcium-binding protein (Scy p 4) that play a significant role in the binding capacity of immunoglobulin G (IgG) and immunoglobulin E (IgE). These linear epitopes were able to inhibit the binding of Scy p 4 to sensitized serum, highlighting their potential diagnostic and food processing applications in shellfish allergy. The findings contribute to a better understanding of the relationship between the structure and allergenicity of Scy p 4.
Sarcoplasmic calcium-binding protein is a stable allergen in Scylla paramamosain and named Scy p 4. To explore the importance of linear epitopes in the immunoglobulin G (IgG)/immunoglobulin E (IgE)-binding capacity of Scy p 4, chemical denaturants were used to destroy the structure. Scy p 4 was reduced with dithiothreitol and subsequently alkylated with iodoacetamide (IAA). Furthermore, the structural analysis indicated that IAA-Scy p 4 was an unstructured protein. The inhibition enzyme-linked immunosorbent assay showed that IAA-Scy p 4 could inhibit the binding of Scy p 4 to sensitize serum, with inhibition rates reached 55%. Moreover, the linear mimotopes of Scy p 4 were predicted in silico. Three linear epitopes were verified by serological tests and named L-Scy p 4-1 (AA(76-91)), L-Scy p 4-2 (AA(111-125)), and L-Scy p 4-3 (AA(137-146)). Overall, these data provide an understanding of the relationship between the structure and allergenicity about Scy p 4, and the identified linear epitopes can be used for diagnosis and food processing of shellfish allergy.
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