Rossellomorea arthrocnemi sp. nov., a novel plant growth-promoting bacterium used in heavy metal polluted soils as a phytoremediation tool

Strain EAR8(T) is a root endophyte isolated from Arthrocnemum macrostachyum plants collected from the Odiel marshes, Huelva (Spain). It presented in vitro plant growth-promoting properties and improved the plant growth and heavy metal accumulation in polluted soils playing an important role in phytoremediation strategies. Phenotypically, strain EAR8(T) cells were Grampositive, aerobic and non-motile rods with terminal oval endospores and non-swollen sporangia which form beige, opaque, butyrous, raised and irregular colonies with undulate margins. The strain was able to grow between 15-45 degrees C, at pH 6.0-9.0 and tolerated 0-25 % NaCl (w/v) showing optimal growth conditions on trypticase soy agar plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 37 degrees C for 24 h. Chemotaxonomic analyses showed that the isolate has meso- diaminopimelic acid as the peptidoglycan in the cell wall and MK 7 as the major respiratory quinone. The predominant fatty acids were anteiso-C-15: 0 and iso-(C15: 0) and the polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on the whole proteomes of closest sequenced relatives confirmed that strain EAR8(T) is affiliated to the genus Rossellomorea and forms a clade with Rossellomorea vietnamensis 15-1(T) with maximum support. Genome analyses showed that EAR8(T) has indole-3-acetic acid and siderophore biosynthesis and transporters genes and genes related to resistance against heavy metals. Phenotypic and phylogenomic comparative studies suggested that strain EAR8(T) is a new representative of the genus Rossellomorea and the name Rossellomorea arthrocnemi sp. nov. is proposed. Type strain is EAR8(T) (=CECT 9072(T)=DSM 103900(T)).

Automated summary

Strain EAR8(T) is a root endophyte isolated from Arthrocnemum macrostachyum plants collected from the Odiel marshes, Huelva (Spain) with the ability to enhance plant growth and heavy metal accumulation. It has Grampositive, aerobic and non-motile rods with specific growth conditions. Phylogenomic studies suggest that strain EAR8(T) belongs to the genus Rossellomorea and a proposed new species Rossellomorea arthrocnemi sp. nov.