Lentilactobacillus fungorum sp. nov., isolated from spent mushroom substrates

In Japan, during a screening of lactic acid bacteria in spent mushroom substrates, an unknown bacterium was isolated and could not be assigned to any known species. Strain YK48G(T) is Gram-stain-positive, rod-shaped, non-motile, non-spore-forming and catalase-negative. The isolate grew in 0-4% (w/v) NaCl, at 15-37 degrees C (optimum, 30 degrees C) and at pH 4.0-8.0 (optimum, pH 6.0). The genomic DNA G+C content of strain YK48G(T) was 42.5 mol%. Based on its 16S rRNA gene sequence, strain YK48G(T) represented a member of the genus Lentilactobacillus and showed the highest pairwise similarity to Lentilactobacillus rapi DSM 19907(T) (97.86%). Phylogenetic analyses based on amino acid sequences of 466 shared protein-encoding genes also revealed that the strain was phylogenetically positioned in the genus Lentilactobacillus but did not suggest an affiliation with previously described species. The average nucleotide identity and digital DNA-DNA hybridization values between strain YK48G(T) and the type strains of phylogenetically related species were 72.2-76.6% and 19.0-21.2%,respectively, indicating that strain YK48G(T) represents a novel species within the genus Lentilactobacillus. Phenotypic data further confirmed the differentiation of strain YK48G(T) from other members of the genus Lentilactobacillus. According to the results of the polyphasic characterization presented in this study, strain YK48G(T) represents a novel species of the genus Lentilactobacillus, for which the name Lentilactobacillus fungorum sp. nov. is proposed. The type strain is YK48G(T) (=JCM 32598(T)=DSM 107968(T)).

Automated summary

A novel bacterium, Lentilactobacillus fungorum sp. nov., was isolated and identified from spent mushroom substrates in Japan. Strain YK48G(T) showed characteristics of being a member of the genus Lentilactobacillus and represented a new species within this genus._phylogenetic analyses supported the differentiation of strain YK48G(T) from previously described species.