Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica

A Gram-stain-positive, aerobic, non-sporulating, yellow-pigmented and rod or cocci-shaped bacterium, designated Arc0846-15(T), was isolated from the kelp Laminaria japonica. Strain Arc0846-15(T) was found to grow at 16-35 degrees C (optimum, 28 degrees C), at pH 6.0-9.5 (optimum, 7.0) and in the presence of 0-6% (w/v) NaCl (optimum, 2%). Cells were positive for catalase and negative for oxidase activity. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, revealed that the nearest phylogenetic neighbour strains of strain Arc0846-15(T) were Ornithinimicrobium murale 01 Gi-040(T) (96.2%), Ornithinimicrobium kibberense K22-20(T) (96.1%) and Ornithinimicrobium humiphilum HKI 0124(T) (95.2%). Based on phylogenomic analysis, the average nucleotide identity values between strain Arc0846-15(T) and the neighbour strains were 69.8, 69.7 and 69.8%, respectively; the digital DNA-DNA hybridization values between strain Arc0846-15(T) and its three closest neighbour strains were 18.8, 19.1 and 19.3%, respectively. The predominant menaquinone was MK-8 (H-4). The dominant cellular fatty acids were C-17:1 omega 8c, iso-C-15:0, iso-C-16:0 and C-17:0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain Arc0846-15(T) was 61.6 mol% based on the whole genome sequence. Based on the phylogenetic and phenotypic characteristics, strain Arc0846-15(T) is considered to represent a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium laminariae sp. nov. is proposed, with Arc0846-15(T) (=KCTC 49655(T)=MCCC 1K06093(T)) as the type strain.

Automated summary

A new bacterium belonging to the genus Ornithinimicrobium was isolated from the kelp Laminaria japonica. It exhibited Gram-positive, aerobic, non-sporulating, yellow-pigmented characteristics.