Hoyosella suaedae sp. nov., a novel bacterium isolated from rhizosphere soil of Suaeda aralocaspica (Bunge) Freitag & Schutze

A Gram-stain-positive, non-motile and coccus-shaped bacterium, designated strain LNNU 331112(T), was isolated from the composite rhizosphere soil of the halophyte Suaeda aralocaspica (Bunge) Freitag and Schutze, which was collected in Xinjiang, northwest China. Growth occurred at 10-45 degrees C, pH 6.0-11.0 and in the presence of 0-10%NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence suggested that strain LNNU 331112(T) belonged to the genus Hoyosella and showed 95.6, 95.5 and 95.4% sequence similarities to Hoyosella altamirensis DSM 45258(T), Hoyosella subflava CGMCC 4.3532(T) and Hoyosella rhizosphaerae CGMCC 1.15478(T), respectively. The estimated digital DNA-DNA hybridization relatedness values between strain LNNU 331112(T) and the type strains of H. altamirensis DSM 45258(T), H. subflava CGMCC 4.3532(T) and H. rhizosphaerae CGMCC 1.15478(T) were 18.9, 19.3 and 18.3%, respectively. The average nucleotide identity values between strain LNNU 331112(T) and H. altamirensis DSM 45258(T), H. subflava CGMCC 4.3532(T) and H. rhizosphaerae CGMCC 1.15478(T) were 72.6, 72.7 and 72.3%, respectively. The genome sequence of strain LNNU 331112(T) showed 69.0-72.3% average amino acid identity values in comparison with the related genome sequences of three validly published Hoyosella species. The genome of strain LNNU 331112(T) was 3.47 Mb, with a DNA G+C content of 68.4 mol%. A total of 3182 genes were identified as protein-coding in strain LNNU 331112(T). Genomic analysis revealed that a number of genes involved in osmotic pressure regulation, intracellular pH homeostasis and potassium (K+) uptake protein were found in strain LNNU 331112(T). The predominant menaquinones were MK-8 (44.6%) and MK-7 (55.4%), which differentiated strain LNNU 331112(T) from other three recognized Hoyosella species. Major fatty acids (>10%) were C-17:1 omega 8c (33.8%), C-16:0 (23.3%), C-17:0 (12.8%) and summed feature 3 (12.9%), which also clearly separated strain LNNU 331112(T) from three recognized Hoyosella species. The polar lipid profile of strain LNNU 331112(T) included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid, one unidentified phospholipid and two unidentified lipids. According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain LNNU 331112(T) is considered to represent a novel species of the genus Hoyosella, for which the name Hoyosella suaedae sp. nov. is proposed. The type strain is LNNU 331112(T) (=KCTC 39808(T)=CGMCC 1.17107(T)=DSM 103463(T)).

Automated summary

A novel Gram-positive bacterium, designated strain LNNU 331112(T), was isolated from the rhizosphere soil of the halophyte Suaeda aralocaspica in Xinjiang, northwest China. Phylogenetic analysis revealed that it belonged to the genus Hoyosella. Genomic and chemotaxonomic analyses supported the proposal of a new species, named Hoyosella suaedae sp. nov., with strain LNNU 331112(T) designated as the type strain.