Brevibacillus marinus sp. nov., a thermophilic bacterium isolated from deep sea sediment in the South China Sea

A novel thermophilic bacterium, designated SCSIO 07484(T) , was isolated from marine sediment sampled in the South China Sea. Growth occurred at 30-60 degrees C, pH 6.0-8.0 and in the presence of 0-3 % (w/v) NaCl. Cells of strain SCSIO 07484(T) were rod-shaped and flagellum-forming. No soluble pigment was observed. The phylogenetic analysis of the 16S rRNA gene sequences indicated that SCSIO 07484(T) belonged to the family Paenibacillaceae and clustered with members of the genus Brevibacillus in the phylogenetic trees with less than 96.2% similarities. The cell wall contained meso-diaminopimelic acid. Whole-cell hydrolysates contained arabinose, glucose and ribose. The predominant menaquinone was MK-7. Major fatty acids were iso-C-16:0, iso-C-15:0, C-16:0 and iso-C-14:0. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine were its diagnostic polar lipids. The whole genome size of strain SCSIO 07484(T) was 4 079826 bp with a DNA G+C content of 56.2mol%, including one circular chromosome of 3 978392 bp and one plasmid of 101434 bp. Based on the polyphasic analysis of strain SCSIO 07484(T), it is considered to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus marinus sp. nov. is proposed with the type strain SCSIO 07484(T) (=DSM 106769(T)=CGMCC 1.15814(T)).

Automated summary

The novel thermophilic bacterium SCSIO 07484(T) was isolated from marine sediment in the South China Sea, showing growth at temperatures of 30-60 degrees C, pH of 6.0-8.0, and in the presence of 0-3% NaCl. Phylogenetic analysis revealed it belonged to the family Paenibacillaceae and clustered with members of the genus Brevibacillus with less than 96.2% similarity.