Journal
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
Volume 28, Issue 2, Pages -Publisher
SPRINGER
DOI: 10.1007/s10989-022-10379-0
Keywords
Locus coeruleus; Orexin-A; Low Ca2+ aCSF; Firing rate; sEPSC
Categories
Funding
- INSF [91059424]
- Tarbiat Modares University
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The noradrenergic neurons of the locus coeruleus (LC) are affected by the orexin-A, which has an excitatory effect on the firing rates of action potentials. This effect is enhanced when the concentration of calcium is low, and it also affects the depolarization and afterhyperpolarization of the neurons. Additionally, orexin-A enhances the frequency of spontaneous excitatory postsynaptic currents (sEPSC) in low external calcium conditions.
Noradrenergic neurons of the locus coeruleus (LC) receive projection from hypothalamus orexinergic neurons and express orexin receptor type 1 (OXR1). Orexin-A has a regulatory effect on feeding, sleep, arousal, reward processing, and drug addiction. Orexinergic neurons make excitatory afferent to the LC neurons. However, the underlying mechanism of the orexin effect on LC neurons is not completely discerned. In the current study, the contribution of calcium to orexin-induced excitation of the rat LC neurons was studied in brainstem slice preparations by the whole-cell patch-clamp method. The application of orexin increased the firing rates of action potentials (APs) in normal artificial cerebrospinal fluid (aCSF). In low Ca2+ aCSF orexin enhanced the firing rate higher than in normal aCSF. Orexin increased the mean amplitude of the depolarization and reduced the afterhyperpolarization (AHP) following each action potential in low Ca2+ aCSF compared to normal aCSF. Furthermore, the frequency of spontaneous excitatory postsynaptic currents (sEPSC) is enhanced by orexin in low external Ca2+ compared to normal aCSF but the amplitude of sEPSCs did not change. Current results have unraveled that orexin-A has an excitatory effect on the rat LC neurons.
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