Functional assessment of miR-1291 in colon cancer cells

miR-1291 exerts an anti-tumor effect in a subset of human carcinomas, including pancreatic cancer. However, its role in colorectal cancer (CRC) is largely unknown. In the present study, the expression and effect of miR-1291 in CRC cells was investigated. It was identified that miR-1291 significantly suppressed the proliferation, invasion, cell mobility and colony formation of CRC cells. Additionally, miR-1291 induced cell apoptosis. A luciferase reporter assay revealed that miR-1291 directly bound the 3 '-untranslated region sequence of doublecortin-like kinase 1 (DCLK1). miR-1291 also suppressed DCLK1 mRNA and protein expression in HCT116 cells that expressed DCLK1. Furthermore, miR-1291 suppressed cancer stem cell markers BMI1 and CD133, and inhibited sphere formation. The inhibitory effects on sphere formation, invasion and mobility in HCT116 cells were also explored and verified using DCLK1 siRNAs. Furthermore, miR-1291 induced CDK inhibitors p21(WAF1/CIP1) and p27(KIP1) in three CRC cell lines, and the overexpression of DCLK1 in HCT116 cells led to a decrease of p21(WAF1/CIP1) and p27(KIP1). Intravenous administration of miR-1291 loaded on the super carbonate apatite delivery system significantly inhibited tumor growth in the DLD-1 xenograft mouse model. Additionally, the resultant tumors exhibited significant upregulation of the p21(WAF1/CIP1) and p27(KIP1) protein with treatment of miR-1291. Taken together, the results indicated that miR-1291 served an anti-tumor effect by modulating multiple functions, including cancer stemness and cell cycle regulation. The current data suggested that miR-1291 may be a promising nucleic acid medicine against CRC.

Automated summary

miR-1291 exerts an anti-tumor effect in colorectal cancer cells by suppressing proliferation, invasion, cell mobility, and colony formation, inducing cell apoptosis, inhibiting cancer stemness, and regulating cell cycle through the modulation of multiple genes and proteins such as DCLK1, BMI1, CD133, p21, and p27.