Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/ijms222011118
Keywords
aptamers; hydrogel beads; Pseudomonas aeruginosa; sepsis
Funding
- Ministry of Science, Research and Arts of the state of Baden-Wurttemberg
- Federal Ministry of Education and Research (BMBF), Biotechnologie 2020+ Basistechnologien: Projekt SeleKomM
- European Union project Horizon 2020 [686271]
- German Science Foundation (DFG) [ZI567/9-1]
- German Federal Ministry of Education and Research (BMBF)
- project executing organization VDI Technologiezentrum GmbH through research project ProMatLeben-Polymers (InGel-NxG) [FKZ: 13XP5086E]
- Ministry of Science, Research and Arts of Baden-Wurttemberg
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Systemic blood stream infections are increasing worldwide, with Pseudomonas aeruginosa being a major multi-resistant pathogen of concern. Early diagnosis of sepsis significantly improves survival chances in septicemia patients. The use of affinity materials like aptamer-functionalized beads for specific pathogen binding in blood shows promising potential for fast and reliable diagnosis of bloodstream infections.
Systemic blood stream infections are a major threat to human health and are dramatically increasing worldwide. Pseudomonas aeruginosa is a WHO-alerted multi-resistant pathogen of extreme importance as a cause of sepsis. Septicemia patients have significantly increased survival chances if sepsis is diagnosed in the early stages. Affinity materials can not only represent attractive tools for specific diagnostics of pathogens in the blood but can prospectively also serve as the technical foundation of therapeutic filtration devices. Based on the recently developed aptamers directed against P. aeruginosa, we here present aptamer-functionalized beads for specific binding of this pathogen in blood samples. These aptamer capture beads (ACBs) are manufactured by crosslinking bovine serum albumin (BSA) in an emulsion and subsequent functionalization with the amino-modified aptamers on the bead surface using the thiol- and amino-reactive bispecific crosslinker PEG(4)-SPDP. Specific and quantitative binding of P. aeruginosa as the dedicated target of the ACBs was demonstrated in serum and blood. These initial but promising results may open new routes for the development of ACBs as a platform technology for fast and reliable diagnosis of bloodstream infections and, in the long term, blood filtration techniques in the fight against sepsis.
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