Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/ijms222413449
Keywords
nephron progenitor cells; direct reprogramming; transdifferentiation; urine cells; kidney
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korean government (MSIT) [2014M3A9D3034158, 2014M3A9D3034164, 2016R1C1B1011180, 2018R1C1B5040264, 2019R1A2C1004046, 2019R1F1A1044473, 2019R1H1A1079839]
- Ministry of Trade, Industry [R0005886]
- School of Life Sciences and Biotechnology for BK21 PLUS, Korea University
- STEMLAB, Inc.
- National Research Foundation of Korea [2014M3A9D3034158] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A novel method has been developed to generate expandable iNPCs from human urine-derived cells, holding broad applicability potential for patient-specific treatment of kidney diseases.
Background: Regenerative medicine strategies employing nephron progenitor cells (NPCs) are a viable approach that is worthy of substantial consideration as a promising cell source for kidney diseases. However, the generation of induced nephron progenitor-like cells (iNPCs) from human somatic cells remains a major challenge. Here, we describe a novel method for generating NPCs from human urine-derived cells (UCs) that can undergo long-term expansion in a serum-free condition. Results: Here, we generated iNPCs from human urine-derived cells by forced expression of the transcription factors OCT4, SOX2, KLF4, c-MYC, and SLUG, followed by exposure to a cocktail of defined small molecules. These iNPCs resembled human embryonic stem cell-derived NPCs in terms of their morphology, biological characteristics, differentiation potential, and global gene expression and underwent a long-term expansion in serum-free conditions. Conclusion: This study demonstrates that human iNPCs can be readily generated and expanded, which will facilitate their broad applicability in a rapid, efficient, and patient-specific manner, particularly holding the potential as a transplantable cell source for patients with kidney disease.
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