Physical Considerations for In Vitro ESWT Research Design

In vitro investigations, which comprise the bulk of research efforts geared at identifying an underlying biomechanical mechanism for extracorporeal shock wave therapy (ESWT), are commonly hampered by inadequate descriptions of the underlying therapeutic acoustical pressure waves. We demonstrate the necessity of in-situ sound pressure measurements inside the treated samples considering the significant differences associated with available applicator technologies and cell containment. A statistical analysis of pulse-to-pulse variability in an electrohydraulic applicator yields a recommendation for a minimal pulse number of n = 300 for cell pallets and suspensions to achieve reproducible treatments. Non-linear absorption behavior of sample holders and boundary effects are shown for transient peak pressures and applied energies and may serve as a guide when in-situ measurements are not available or can be used as a controllable experimental design factor. For the use in microbiological investigations of ESWT we provide actionable identification of common problems in describing physical shockwave parameters and improving experimental setups by; (1) promoting in-situ sound field measurements, (2) statistical evaluation of applicator variability, and (3) extrapolation of treatment parameters based on focal and treatment volumes.

Automated summary

In vitro investigations on extracorporeal shock wave therapy (ESWT) often lack adequate descriptions of therapeutic acoustical pressure waves. In this study, we demonstrate the necessity of in-situ sound pressure measurements inside treated samples and provide recommendations for reproducible treatments. We also discuss the non-linear absorption behavior and boundary effects, which can serve as a guide in the absence of in-situ measurements. For microbiological investigations of ESWT, we offer actionable suggestions to improve experimental setups.