Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/ijms23010002
Keywords
physiological ovarian aging; histone modifications; female reproductive functions; post-translational modifications; mice
Funding
- National Natural Science Foundation of China [82160284, 82071708, 81771583]
- Major Academic and Technical Leaders Training Program of Jiangxi Province [20194BCJ22005]
- Natural Science Foundation of Jiangxi province [20171ACB21006, 20202ACB216003]
- Special Funds for Central Government to Guide Local Scientific and Technological Development [20202ZDB01013]
- Double-Thousand Talents Plan of Jiangxi Province [jxsq2019201091]
- Special Fund of Innovation for Post-graduate Students in Nanchang University [CX2019154]
- Key projects of the Science and Technology Plan of Jiangxi Provincial Administration of Traditional Chinese Medicine [2021Z019]
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This study found that altered regulatory enzymes and post-translational modifications (PTMs) are associated with physiological ovarian aging in mice. These findings are expected to provide useful insights for delaying ovarian aging and the diagnosis and treatment of female infertility.
Post-translational modifications (PTMs) have been confirmed to be involved in multiple female reproductive events, but their role in physiological ovarian aging is far from elucidated. In this study, mice aged 3, 12 or 17 months (3M, 12M, 17M) were selected as physiological ovarian aging models. The expression of female reproductive function-related genes, the global profiles of PTMs, and the level of histone modifications and related regulatory enzymes were examined during physiological ovarian aging in the mice by quantitative real-time PCR and western blot, respectively. The results showed that the global protein expression of Kbhb (lysine beta-hydroxybutyryllysine), Khib (lysine 2-hydroxyisobutyryllysine), Kglu (lysineglutaryllysine), Kmal (lysinemalonyllysine), Ksucc (lysinesuccinyllysine), Kcr (lysinecrotonyllysine), Kbu (lysinebutyryllysine), Kpr (lysinepropionyllysine), SUMO1 (SUMO1 modification), ub (ubiquitination), P-Typ (phosphorylation), and 3-nitro-Tyr (nitro-tyrosine) increased significantly as mice aged. Moreover, the modification level of Kme2 (lysinedi-methyllysine) and Kac (lysineacetyllysine) was the highest in the 3M mice and the lowest in 12M mice. In addition, only trimethylation of histone lysine was up-regulated progressively and significantly with increasing age (p < 0.001), H4 ubiquitination was obviously higher in the 12M and 17M mice than 3M (p < 0.001), whereas the modification of Kpr (lysinepropionylation) and O-GlcNA in 17M was significantly decreased compared with the level in 3M mice (p < 0.05, p < 0.01). Furthermore, the expression levels of the TIP60, P300, PRDM9, KMT5B, and KMT5C genes encoding PTM regulators were up-regulated in 17M compared to 3M female mice (p < 0.05). These findings indicate that altered related regulatory enzymes and PTMs are associated with physiological ovarian aging in mice, which is expected to provide useful insights for the delay of ovarian aging and the diagnosis and treatment of female infertility.
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