Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/ijms222212183
Keywords
phase-inversion technique; vascular prosthesis; polyurethane; surface modification; hemocompatibility; endothelial cell; REDV; YIGSR
Funding
- National Centre for Research and Development [LIDER/18/0104/L-8/16/NCBR/2017]
Ask authors/readers for more resources
This study describes a method for modifying polyurethane small-diameter vascular prostheses by introducing acrylic acid and peptides to increase EC adhesion and decrease platelet adhesion, resulting in materials with high hemocompatibility._comparison of two types of peptide coatings showed that YISGR-coated prostheses had more promising results than REDV-coated ones.
This study describes a method for the modification of polyurethane small-diameter (5 mm) vascular prostheses obtained with the phase inversion method. The modification process involves two steps: the introduction of a linker (acrylic acid) and a peptide (REDV and YIGSR). FTIR and XPS analysis confirmed the process of chemical modification. The obtained prostheses had a porosity of approx. 60%, Young's Modulus in the range of 9-11 MPa, and a water contact angle around 40 & DEG;. Endothelial (EC) and smooth muscle (SMC) cell co-culture showed that the surfaces modified with peptides increase the adhesion of ECs. At the same time, SMCs adhesion was low both on unmodified and peptide-modified surfaces. Analysis of blood-materials interaction showed high hemocompatibility of obtained materials. The whole blood clotting time assay showed differences in the amount of free hemoglobin present in blood contacted with different materials. It can be concluded that the peptide coating increased the hemocompatibility of the surface by increasing ECs adhesion and, at the same time, decreasing platelet adhesion. When comparing both types of peptide coatings, more promising results were obtained for the surfaces coated with the YISGR than REDV-coated prostheses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available