Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/ijms23042063
Keywords
callose; callose synthase; heat; root meristem; plasmodesmata; thermotolerance
Funding
- National Natural Science Foundation of China [32070192]
- Scientific Research Foundation for Advanced Talents of Henan University
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This study reveals that heat stress induces callose accumulation at the plasmodesmata connecting sieve element and phloem pole pericycle, resulting in decreased root meristem size and inhibition of phloem unloading. It further identifies CALLOSE SYNTHASE 8 (CalS8) as a negative regulator of thermotolerance in Arabidopsis roots by decreasing plasmodesmal callose deposition.
The intercellular transport of sugars, nutrients, and small molecules is essential for plant growth, development, and adaptation to environmental changes. Various stresses are known to affect the cell-to-cell molecular trafficking modulated by plasmodesmal permeability. However, the mechanisms of plasmodesmata modification and molecules involved in the phloem unloading process under stress are still not well understood. Here, we show that heat stress reduces the root meristem size and inhibits phloem unloading by inducing callose accumulation at plasmodesmata that connect the sieve element and phloem pole pericycle. Furthermore, we identify the loss-of-function of CALLOSE SYNTHASE 8 (CalS8), which is expressed specifically in the phloem pole pericycle, decreasing the plasmodesmal callose deposition at the interface between the sieve element and phloem pole pericycle and alleviating the suppression at root meristem size by heat stress. Our studies indicate the involvement of callose in the interaction between root meristem growth and heat stress and show that CalS8 negatively regulates the thermotolerance of Arabidopsis roots.
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