Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/ijms222413649
Keywords
hemoglobin; oxidative modification; dinitrosyl iron complexes; peroxynitrite; carbonyl derivatives
Funding
- Russian Foundation for Basic Research [19-29-12052, 19-015-00444]
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DNICs, a physiological form of nitric oxide in organisms, act as antioxidants and antiradical agents to protect hemoglobin from oxidative modification caused by peroxynitrite through intercepting peroxynitrite and free radicals.
Dinitrosyl iron complexes (DNICs) are a physiological form of nitric oxide ((NO)-N-center dot) in an organism. They are able not only to deposit and transport (NO)-N-center dot, but are also to act as antioxidant and antiradical agents. However, the mechanics of hemoglobin-bound DNICs (Hb-DNICs) protecting Hb against peroxynitrite-caused, mediated oxidative modification have not yet been scrutinized. Through EPR spectroscopy we show that Hb-DNICs are destroyed under the peroxynitrite action in a dose-dependent manner. At the same time, DNICs inhibit the oxidation of tryptophan and tyrosine residues and formation of carbonyl derivatives. They also prevent the formation of covalent crosslinks between Hb subunits and degradation of a heme group. These effects can arise from the oxoferryl heme form being reduced, and they can be connected with the ability of DNICs to directly intercept peroxynitrite and free radicals, which emerge due to its homolysis. These data show that DNICs may ensure protection from myocardial ischemia.
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