Bee Venom Activates the Nrf2/HO-1 and TrkB/CREB/BDNF Pathways in Neuronal Cell Responses against Oxidative Stress Induced by Aβ1-42

Honeybee venom has recently been considered an anti-neurodegenerative agent, primarily due to its anti-inflammatory effects. The natural accumulation of amyloid-beta (A beta) in the brain is reported to be the natural cause of aging neural ability downfall, and oxidative stress is the main route by which A beta ignites its neural toxicity. Anti-neural oxidative stress is considered an effective approach for neurodegenerative therapy. To date, it is unclear how bee venom ameliorates neuronal cells in oxidative stress induced by A beta. Here, we evaluated the neuroprotective effect of bee venom on A beta-induced neural oxidative stress in both HT22 cells and an animal model. Our results indicate that bee venom protected HT22 cells against apoptosis induced by A beta(1-42). This protective effect was explained by the increased nuclear translocation of nuclear factor erythroid 2-like 2 (Nrf2), consequently upregulating the production of heme oxygenase-1 (HO-1), a critical cellular instinct antioxidant enzyme that neutralizes excessive oxidative stress. Furthermore, bee venom treatment activated the tropomyosin-related kinase receptor B (TrkB)/cAMP response element-binding (CREB)/brain-derived neurotrophic factor (BDNF), which is closely related to the promotion of cellular antioxidant defense and neuronal functions. A mouse model with cognitive deficits induced by A beta(1-42) intracerebroventricular (ICV) injections was also used. Bee venom enhanced animal cognitive ability and enhanced neural cell genesis in the hippocampal dentate gyrus region in a dose-dependent manner. Further analysis of animal brain tissue and serum confirmed that bee venom reduced oxidative stress, cholinergic system activity, and intercellular neurotrophic factor regulation, which were all adversely affected by A beta(1-42). Our study demonstrates that bee venom exerts antioxidant and neuroprotective actions against neural oxidative stress caused by A beta(1-42,) thereby promoting its use as a therapeutic agent for neurodegenerative disorders.

Automated summary

Honeybee venom has antioxidant and neuroprotective effects, alleviating neural oxidative stress caused by amyloid-beta and enhancing cognitive ability and neural cell genesis. Therefore, honeybee venom is of significant importance in the treatment of neurodegenerative disorders.