Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/ijms23020746
Keywords
pear (Pyrus communis); glutathione S-transferase; anthocyanins; PcGST57
Funding
- National Natural Science Foundation of China [31971691]
- China Agriculture Research System of MOF
- MARA, Central Public-Interest Scientific Institution Basal Research Fund [1610192021302]
- Collaborative Innovation Project of ZFRI, CAAS [ZGS202107]
- Agricultural Science and Technology Innovation Program (CAAS-ASTIP), National Key R&D Program of China [2019YFD1001401]
- Natural Science Foundation of Henan Province, China [202300410556]
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In this study, a total of 57 PcGST genes involved in anthocyanin accumulation in pear peels were identified through genomic analysis. Glutamic acid was found to significantly improve anthocyanin accumulation, with PcGST57 being the most significantly induced gene. Further experiments showed that overexpression of PcGST57 increased anthocyanin accumulation, while silencing of PcGST57 reduced anthocyanin content. These findings provide candidate genes for red pear breeding.
Anthocyanin accumulation in vacuoles results in red coloration in pear peels. Glutathione S-transferase (GST) proteins have emerged as important regulators of anthocyanin accumulation. Here, a total of 57 PcGST genes were identified in the European pear 'Bartlett' (Pyrus communis) through comprehensive genomic analysis. Phylogenetic analysis showed that PcGST genes were divided into 10 subfamilies. The gene structure, chromosomal localization, collinearity relationship, cis-elements in the promoter region, and conserved motifs of PcGST genes were analyzed. Further research indicated that glutamic acid (Glu) can significantly improve anthocyanin accumulation in pear peels. RNA sequencing (RNA-seq) analysis showed that Glu induced the expression of most PcGST genes, among which PcGST57 was most significantly induced. Further phylogenetic analysis indicated that PcGST57 was closely related to GST genes identified in other species, which were involved in anthocyanin accumulation. Transcript analysis indicated that PcGST57 was expressed in various tissues, other than flesh, and associated with peel coloration at different developmental stages. Silencing of PcGST57 by virus-induced gene silencing (VIGS) inhibited the expression of PcGST57 and reduced the anthocyanin content in pear fruit. In contrast, overexpression of PcGST57 improved anthocyanin accumulation. Collectively, our results demonstrated that PcGST57 was involved in anthocyanin accumulation in pear and provided candidate genes for red pear breeding.
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