Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Heat stress (HS) is a prevalent negative factor affecting plant growth and development, as it is predominant worldwide and threatens agriculture on a large scale. PHYTOCHROMES (PHYs) are photoreceptors that control plant growth and development, and the stress signaling response partially interferes with their activity. PHYA, B1, and B2 are the most well-known PHY types in tomatoes. Our study aimed to identify the role of tomato 'Money Maker' phyA and phyB1B2 mutants in stable and fluctuating high temperatures at different growth stages. In the seed germination and vegetative growth stages, the phy mutants were HS tolerant, while during the flowering stage the phy mutants revealed two opposing roles depending on the HS exposure period. The response of the phy mutants to HS during the fruiting stage showed similarity to WT. The most obvious stage that demonstrated phy mutants' tolerance was the vegetative growth stage, in which a high degree of membrane stability and enhanced water preservation were achieved by the regulation of stomatal closure. In addition, both mutants upregulated the expression of heat-responsive genes related to heat tolerance. In addition to lower malondialdehyde accumulation, the phyA mutant enhanced proline levels. These results clarified the response of tomato phyA and phyB1B2 mutants to HS.

Automated summary

Heat stress is a prevalent negative factor affecting plant growth and development. In this study, the response of tomato phyA and phyB1B2 mutants to high temperatures at different growth stages was investigated. The mutants showed tolerance to heat stress during the seed germination and vegetative growth stages, while their response during the flowering stage was dependent on the duration of heat exposure. The vegetative growth stage demonstrated the highest tolerance, with increased membrane stability and water preservation achieved through stomatal closure regulation. Additionally, both mutants upregulated heat-responsive genes and the phyA mutant showed enhanced proline levels and lower malondialdehyde accumulation.