High sensitive sol-gel based electrochemical immunosensor for Clenbuterol Determination

Clenbuterol is a class of sympathomimetic agents that are used clinically to treat asthma and bronchospasm. It also has physiological effects similar to those of metabolic steroids to promote the growth of muscle tissue, and is therefore abused in animal husbandry to improve lean muscle mass. It is also used as an agonist that has been banned in sports. In this work, a sensitive electrochemical immunosensor was constructed by modifying ZnO nanoparticles and clenbuterol onto the electrode surface using sol-gel method with clenbuterol being the detection target. Under the optimized experimental conditions, the immunosensor showed a wide linear range and low detection limit. The working linear range was 0.3 to 1000 ng/mL, and the detection limit was 0.12 ng/mL. Meanwhile, this immunosensor is simple, rapid, reproducible and stable. In addition, the above sensor has a good accuracy and immunity to interference in the detection of animal feed samples.

Automated summary

In this study, a sensitive electrochemical immunosensor was built using sol-gel method to detect clenbuterol. The sensor exhibited a wide linear range, low detection limit, simplicity, and stability. It also showed good accuracy and interference immunity in the detection of animal feed samples.