Short-time deep eutectic solvents pretreatment enhanced production of fermentable sugars and tailored lignin nanoparticles from abaca

Deep eutectic solvents (DES) pretreatment is a promising approach to decrease biomass recalcitrance and boost the cellulose bioconversion as well as lignin valorization. In this study, a short-time DES pretreatment strategy was performed to enhance the production of high-yield fermentable sugars and tailored lignin nanoparticles (LNPs) from abaca. The glucose yield reached 92.4% under the optimal pretreatment condition (110 degrees C, 30 min), which was dramatically increased in comparison with that (9.5%) of control abaca. Simultaneously, nuclear magnetic resonance (NMR) and gel permeation chromatography (GPC) techniques indicated that the removed and regenerated DES lignin fractions displayed depolymerized structures and have relatively low molecular weight with relatively homogeneous morphology and narrow size distribution. Transmission electron microscope (TEM) analysis indicated that these lignin fractions are LNPs and the size of the optimal LNPs fraction is ranged from 30 nm to 50 nm. Moreover, all the DES lignin exhibited excellent antioxidant activities as compared to the commercial antioxidant butylated hydroxytoluene (BHT), which can be used as a promising natural antioxidant in industry. In short, this study demonstrated that the short-time DES pretreatment will improve the enzymatic digestibility and facilitate the controllable production and valorization of LNPs from abaca biomass, which will further promote the economic and overall benefits of biorefinery.

Automated summary

The study demonstrates that short-time DES pretreatment is effective in producing high-yield fermentable sugars and tailored LNPs from abaca. Analysis using NMR and GPC showed that DES lignin fractions have depolymerized structures with relatively low molecular weight, and TEM analysis confirmed that these lignin fractions are LNPs. Furthermore, all DES lignin exhibited excellent antioxidant activities, making them potential natural antioxidants for industrial applications.