Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 188, Issue -, Pages 729-739Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.08.053
Keywords
Polysaccharide; Immunomodulatory activity; Structure characterization; NMR; Pumpkin; Macrophages
Funding
- opening fund of Beijing Advanced Innovation Center for Food Nutrition and Human Health
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A neutral polysaccharide CMDP-1a was isolated from pumpkin, with a determined structure of alpha-1,4 linked glucopyranosyl residues. This polysaccharide showed potential immunomodulatory effects and could be used as a food immunomodulator.
A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography. On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of alpha-1,4 linked glucopyranosyl residues with alpha-Glcp residue linkage at backbone C-6. Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution. In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity. Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages. These results indicated that CMDP-1a might be a potential immunomodulator for food purposes.
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