Composition and structure elucidation of bovine milk glycosaminoglycans and their anti-adipogenic activity via modulation PPAR-γ and C/EBP-α

The structure function relation of Glycosaminoglycans from bovine milk (bmGAGs) has not been studied in detail. In the present study bmGAGs was isolated and structurally characterized. Chondroitin sulphate was one of the major GAGs present and had 65% of Delta Di-diSB (GlcA(2S)-GalNAc(4S)), followed by 18% of Delta Di-4S (Delta 4,5HexUA alpha 1 -> 3GalNAc). Further, bmGAGs exhibited a marked anti-adipogenic effect in 3 T3-L1 cells without affecting cell viability at the concentration used. The triglyceride content treated with bmGAGs was significantly decreased as assessed by Oil-Red O staining. Peroxisome proliferator activated receptor gamma (PPAR-gamma) and CCAAT/enhancer-binding proteins (C/EBP alpha) the critical transcription factors in adipogenesis showed significant decrease in both gene and protein levels. Sterol regulatory element-binding protein 1c (SREBP-1c) that promotes the adipocyte differentiation by enhancing the activity of PPAR-gamma was inversely affected by bmGAGs and the fatty acid synthase (FAS) expression was modulated. Thus, the present work is among the firsts to demonstrate an anti-adipogenic activity of bmGAGs by modulating the adipogenesis-related marker proteins and hence bmGAGs may be used as a supplement/therapeutic in the management of obesity.

Automated summary

The study demonstrated the anti-adipogenic activity of bovine milk Glycosaminoglycans (bmGAGs) by modulating adipogenesis-related marker proteins, suggesting a potential therapeutic use of bmGAGs in the management of obesity.