Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 193, Issue -, Pages 81-87Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.10.082
Keywords
Amylomaltase; Glycogen debranching enzyme; Large-ring cyclodextrin; Starch
Funding
- Chulalongkorn University
- 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund)
- Program Management Unit for Human Resources & Institutional Development, Research and Innovation, NXPO [B05F630025]
- Structural and Computational Biology Research Unit
- C2F Postdoctoral Fellowship
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Using CgGDE pretreatment of starch can enhance the production of LR-CDs, significantly improve debranching efficiency and yield, while reducing the generation of by-products.
Synthesis of large-ring cyclodextrins (LR-CDs) in any significant amount has been challenging. This study enhanced the LR-CDs production by Thermus filiformis amylomaltase (TfAM) enzyme by starch pretreatment using glycogen debranching enzyme from Corynebacterium glutamicum (CgGDE). CgGDE pretreated tapioca starch gave LR-CD conversion of 31.2 +/- 2.2%, compared with LR-CDs produced from non-treated tapioca starch (16.0 +/- 2.4%). CgGDE pretreatment enhanced amylose content by approximately 30%. Notably, a shorter incubation time of 1 h is sufficient for CgGDE starch pretreatment to produce high LR-CD yield, compared with 6 h required for the commercial isoamylase. High-Performance Anion Exchange Chromatography coupled with Pulsed Ampemmetric Detection (HPAEC-PAD) and Gel Permeable Chromatography (GPC) revealed that CgGDE is more efficient than the commercial isoamylase in debranching tapioca starch and gave lower molecular weight products. In addition, lower amount of by-products (linear oligosaccharides) were detected in cyclization reaction when using CgGDE-pretreated starch. In conclusion, CgGDE is a highly effective enzyme to promote LR-CD synthesis from starch with a shorter incubation time than the commercial isoamylase.
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