Journal
INTEGRATIVE CANCER THERAPIES
Volume 21, Issue -, Pages -Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/15347354211069397
Keywords
breast precancerous lesions; angiogenesis; Ras; Raf; MEK; ERK; miR-21; Ruyan Neixiao Cream(RUc)
Categories
Funding
- National Natural Science Foundation of China [82074430, 81803979, 81741130, 81673979, 81473688, 81373314]
- Natural Science Foundation of Guangdong Province, China [2018A030313393, 2016A030313114]
- Natural Science Foundation of Guangdong Province, China
- Science and Technology Program of Guangzhou, China [201803010051, 201707010245, 201704020117]
- Fourth Batch of TCM Clinical Outstanding Talent Program of China [444258]
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Ruyan Neixiao Cream (RUc), a traditional Chinese herbal formula, can inhibit angiogenesis of breast precancerous lesions by inhibiting the Ras/Raf/MEK/ERK signaling pathway. Experimental results showed that RUc significantly inhibited cell proliferation, migration, and invasion, and reduced the secretion of angiogenic factors.
Ruyan Neixiao Cream (RUc) is a traditional Chinese herbal formula which can effectively inhibit the angiogenesis of breast precancerous lesions. In order to reveal the specific mechanism, we carried out experiments in vitro and in vivo. We found that the conditioned medium of MCF-10AT cells treated with RUc transdermal solution (RUt) could significantly inhibit the proliferation, migration, invasion, tube formation of HUVECs and the capillary formation of rat aortic rings. RUt may down-regulate the expression of VEGF, MMP2, and MMP9 in MCF-10AT medium by down-regulating miR-21 and up-regulating TIMP-3 and RECK. We further confirmed in rats that the microvascular density of precancerous lesions decreased significantly after external use of RUc, which may be related to the inhibition of Ras/Raf/MEK/ERK signaling pathway related proteins. Presumptively, RUc may inhibit the angiogenesis of breast precancerous lesions by inhibiting Ras/Raf/MEK/ERK signaling pathway, thus relieving the inhibition of miR-21 on TIMP-3 and RECK, then down-regulating the secretion of angiogenic factors.
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