Pulsed electric field treatment enhances lipid bioaccessibility while preserving oxidative stability in Chlorella vulgaris

There is growing demand for gentle technologies to improve the lipid bioaccessibility (BA) of Chlorella vulgaris biomass while preserving cell integrity and therefore oxidative stability. Pulsed electric field treatment (PEF, 5 mu s at 20 kV cm(-1), 31.8 kJ kg(sus)(-1)) led to an enhancement in lipid BA from 4-7.8% (untreated) to 18.7-20.9%. To reach such a level of BA, incubation in buffer after the treatment (12 h at 25/37 degrees C, 48 h at 4 degrees C) was required. As hypothesized, PEF preserved cell integrity, as shown by particle size and scanning electron microscopy analyses, as well as oxidative stability of the biomass over 3 months at 40 degrees C. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future work should focus on further understanding the mechanism behind incubation after PEF and studying the potential effect played by endogenous cell wall-degrading enzymes.

Automated summary

The study demonstrated that pulsed electric field treatment can enhance lipid bioaccessibility of Chlorella vulgaris while maintaining cell integrity and oxidative stability, requiring incubation post-treatment. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future research should focus on further understanding the mechanism behind incubation after PEF and the potential effect played by endogenous cell wall-degrading enzymes.