Photodynamic Inactivation of Bacteria In Vitro Under the Effect of Blue Light

We studied the photodynamic inactivation of E. coli, S. aureus, and P. aeruginosa in vitro. Bacteria suspensions were incubated with riboflavin and exposed to blue light emitted by a diode matrix. Exogenous photosensitizer riboflavin efficiently inactivated bacteria, while methylene blue was ineffective. Under the effect of blue light in a dose of 117 J/cm(2), photoinactivation of E. coli, S. aureus, and P. aeruginosa occurred without photosensitizer riboflavin.