Journal
INFECTION GENETICS AND EVOLUTION
Volume 95, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.meegid.2021.105059
Keywords
Glanders; India; Pakistan; Epidemiology; WGS; SNP markers; PCR-HRM
Categories
Funding
- European Commission's Directorate-General for Health and Consumers
- Indian Council of Agricultural Research, New Delhi, India
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The study sequenced 10 B. mallei strains isolated from India and identified four new markers for tracking and differentiating strains, which will contribute to the epidemiological monitoring of glanders infections, especially in endemic regions such as South Asia and the Middle East.
Glanders, caused by a bacterium called B. mallei, is primarily an infectious horse and human disease. Although its incidence is rare in developed countries, it is nonetheless prevalent in several geographical areas of the world. There is a lack of cost-effective, rapid and specific molecular typing tools for epidemiological tracing of glanders cases. We previously reported an SNP-based typing method that categorizes global B. mallei strains into three lineages (L1 to L3), as well as additional branches, sub-branches and groups. However, further discrimination of the Indian and Pakistani isolates within the L2B2sB2 sub-branch was not possible due to the lack of sufficient epidemiological markers. In this study, 10 B. mallei strains isolated from four states in India during 2015-2016 were whole genome sequenced; SNP analysis further confirmed their position in the L2B2sB2 branch. To better track the strains, four new markers targeting Indian or Pakistani strains, and specifically targeting sub-groups within the Indian strains, were identified. The new SNP markers were tested and validated on the 10 Indian isolates included in this study as well as on 6 contemporary B. mallei Pakistani strains. These rapid and discriminating typing tools will contribute to the epidemiological monitoring of B. mallei infections, particularly in South Asia and the Middle East, endemic regions of the disease.
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