Polyphenols from Artemisia argyi leaves: environmentally friendly extraction under high hydrostatic pressure and biological activities

In this work, high hydrostatic pressure-assisted extraction (HPE) was applied in polyphenol extraction from Artemisia argyi leaf (AAL). The optimal HPE conditions (liquid:solid ratio of 33 mL/g, extraction time of 34 min, high hydrostatic pressure of 301 MPa, and temperature of 71 degrees C) were identified through response surface methodology. The above optimal HPE conditions provided the maximum extraction yield of 64.96 mg/g. A. leaf polyphenol (AALP) was found to be a typical mixture of five phenolic acids, namely, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid, and isochlorogenic acid C. Moreover, AALP exhibited superior antioxidant activity in a concentration-dependent manner in vitro. The 1,1diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) hydroxyl radical scavenging and reducing abilities of AALP obtained through HPE increased remarkably compared with those of the control group. In addition, in the inhibition of biofilm formation by all the test strains, the AALP group exhibited perfect performance that was considerably better than the performance of the control group. In terms of antitumor activity, AALP acquired through HPE revealed a higher inhibitory activity than the control group, and both groups had a better inhibitory effect on HeLa cells than on HCT116 cells. HPE presented higher extraction yields and efficiency than traditional extraction and could therefore be recommended as a state-of-the-art environmentally friendly technology for the extraction of polyphenols from plant sources.

Automated summary

The study successfully utilized high hydrostatic pressure-assisted extraction (HPE) to extract polyphenols from Artemisia argyi leaf, resulting in a high extraction yield and efficiency. The extracted polyphenols displayed strong antioxidant properties, inhibition of biofilm formation, and antitumor activity, making HPE a promising environmentally friendly technology for polyphenol extraction from plant sources.