Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 171, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.indcrop.2021.113891
Keywords
Haplotype-based selection; Wood quality and yield; Epistasis; Multi-omics; Elite trees; Populus
Categories
Funding
- Project of the National Natural Science Foundation of China [31872707, 31500550, 31872671]
- Natural Science Foundation of Beijing Municipality [6212021]
- Fok Ying Tung Education Foundation of China [171020]
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Genetic improvement of woody bioenergy crops is crucial for maximizing industrial product yield, but conventional methods are slow. This study identified key genes involved in lignocellulosic biosynthesis in Populus, providing important insights for future genetic enhancement efforts.
Genetic improvement of woody bioenergy crops is essential to maximize the genetic gain of industrially useful products. Conventional selective breeding to improve complex traits is laborious and time-consuming. A molecular marker-assisted selection approach based on multi-omics genetic dissection and pyramiding gene module utilization has not been developed for woody industrial crops. We initially identified 80 correlated genes enriched in co-expression modules that sustainably participate in lignocellulosic biosynthesis during various developmental periods in Populus. Using an adult germplasm population (15 years old, 435 accessions) of Populus tomentosa, we integrated association mapping, expression quantitative trait loci, and epistasis analyses to reveal the pleiotropy of causative genes within the core co-expression modules, such as ALG14, GSL8, SMT1, and IRX15-L.2, that drive natural variations in gene expression and harvested wood traits. We further pyramided two superior haplotypes and one desirable epistatic allele (Hap_01(PtoSMT1)+Hap_01(PtoALG14)+SNP2(PtoIRX15)-L.(2)) to alleviate linkage drag in multi-trait selection and improve industrial pulpwood products. Early-period selection study and genetic interpretation of the allelic differences of candidate elite trees in a juvenile germplasm population (5 years old, 435 accessions) demonstrated the accuracy and efficiency of our haplotype-based selection method relative to phenotype-based selection. This study provides novel insights into the improvement of industrial wood traits and also facilitates the rational breeding of perennial trees.
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